Undifferentiated Human Adipose Tissue–Derived Stromal Cells Induce Mandibular Bone Healing in Rats
Claudio Parrilla, MD, PhD; Nathalie Saulnier, PhD; Camilla Bernardini, PhD; Riccardo Patti, MS; Tommaso Tartaglione, MD; Anna Rita Fetoni, MD, PhD; Enrico Pola, MD, PhD; Gaetano Paludetti, MD; Fabrizio Michetti, MD; Wanda Lattanzi, MD, PhD
Arch Otolaryngol Head Neck Surg. 2011;137(5):463-470. doi:10.1001/archoto.2011.61
Objective To test the osteo-regenerative potential of adipose tissue–derived stromal cells (ATSCs), an attractive human source for tissue engineering, in a rat model of mandibular defect. Human dermal fibroblasts (HDFs) were used as a differentiated cellular control in the study.
Design The ATSCs and HDFs were isolated from human lipoaspirate and skin biopsy specimens, respectively. Cells were characterized in vitro and then adsorbed on an osteo-conductive scaffold to be transplanted in a mandibular defect of immunosuppressed rats. Naked unseeded scaffold was used as a negative control.
Main Outcome Measures Bone healing was studied by computerized tomography and histologic analysis after 4, 8, and 12 weeks.
Results Computed tomography showed that undifferentiated ATSCs induced successful bone healing of the mandible defect when transplanted in animals, compared with HDFs and negative controls. Histologic analysis demonstrated that the newly formed tissue in the surgical defect retained the features of compact bone.
Conclusion Undifferentiated human ATSCs are suitable for cell-based treatment of mandibular defects, even in the absence of previous osteogenic induction in vitro.
Author Affiliations: Departments of Otolaryngology (Drs Parrilla, Fetoni, and Paludetti), Internal Medicine (Dr Saulnier), Radiology (Dr Tartaglione), Orthopaedics (Dr Pola), and Institute of Anatomy and Cell Biology (Drs Bernardini, Michetti, and Lattanzi and Mr Patti), Universit? Cattolica del Sacro Cuore, School of Medicine A. Gemelli, Rome, Italy; INSERM, Paris, France (Dr Saulnier); and Latium Muscular Skeletal Tissue Bank, Rome, Italy (Dr Michetti).
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